Biswajit Chatterjee was born on 14 December 1936 in Calcutta, Bengal Presidency, British India, now Kolkata, West Bengal. He began his acting career in Bengali cinema before moving to Bombay and establishing himself as a leading actor in Hindi films.
He gained major recognition in Hindi cinema with Bees Saal Baad (1962), which became a significant success. He subsequently appeared in several popular films, including Shehnai, Mere Sanam, Kohraa, Yeh Raat Phir Naa Ayegi, April Fool, Do Kaliyan, and Night in London.
Biswajit was particularly known for his romantic roles and was paired with leading actresses including Asha Parekh, Mala Sinha, Mumtaz, and Rajshree. His films were also known for their memorable music, which contributed to his popularity during the 1960s.
Apart from acting, Biswajit also worked as a producer, director, and singer. He directed and produced Kahtey Hain Mujhko Raja (1975), with music composed by R. D. Burman. He is also the father of Bengali actor Prosenjit Chatterjee.