Prem Chopra was born on 23 September 1935 in Lahore, Punjab, British India, now in Pakistan. He was raised in Shimla after the Partition of India. His father, Ranbirlal Chopra, worked at the Attorney-General's office. Prem Chopra graduated in arts from Punjab University and developed an interest in acting through theatre during his college years.
Before entering films, he worked in the circulation department of The Times of India in Mumbai while trying to establish himself as an actor. His early work included Punjabi cinema, and he appeared in Chaudhary Karnail Singh. His first Hindi film was Mud Mud Ke Na Dekh (1960).
His breakthrough came with films such as Shaheed (1965), Woh Kaun Thi? (1964), Upkar (1967), Do Raaste (1969), Kati Patang (1970), Bobby (1973), Do Anjaane (1976), Kranti (1981), and Dostana (1980). His villainous performances made him one of the most sought-after antagonists of the 1970s and 1980s.
Although he became famous for negative roles, Prem Chopra also played positive and character roles throughout his career. His later work includes films such as Koi... Mil Gaya, Bunty Aur Babli, Rocket Singh: Salesman of the Year, Delhi-6, and Animal.
Prem Chopra received the Filmfare Lifetime Achievement Award in 2023 for his contribution to Indian cinema. He has remained associated with the film industry well into his later years.